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. 2013 Apr 18;4(4):e603. doi: 10.1038/cddis.2013.108

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Copyright © 2013 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Apoptosis following mitotic catastrophe induced by knockdown of centrosomal proteins depends on Chk2. (a) Levels of Chk2 and its phosphorylated form (p-Chk2) in HeLa cells transfected with siRNA for centrosomal proteins. Lysates obtained 48 h after siRNA transfection were analyzed by western blotting. (b) Subcellular localization of Chk2 and p-Chk2 in siRNA-transfected cells 48 h after transfection. Bar, 20 μm. (c) The level of Chk2 in HeLa cells cotransfected with GL3 control siRNA and siRNA against Chk2 was analyzed by western blotting 48 h after siRNA transfection. (d) Rates of cell death (expressed as percentages) induced by simultaneous knockdown of centrosomal proteins and Chk2. At least 200 randomly selected cells were counted 48 h after siRNA transfection. Values are an average of three independent experiments. Error bars represent S.D.