Figure 7.

(a) Immunoblot analysis showing successful knockdown of CRYAB and significant increase in the ratio of cytosolic/mitochondrial cytochrome c in myocytes transfected with siRNA against CRYAB during induction of hypoxia. This siRNA treatment had no effect on the level of cytosolic cytochrome c in myocytes treated with Ang II. (b) Graph showing ratio of cytosolic/mitochondrial cytochrome c in control, AngII-treated or hypoxic myocytes transfected with either siRNA against CRYAB or non-specific siRNA (**P<0.01). (c) Graph showing caspase 3 activity in the three experimental groups (Control, Ang II and hypoxia) treated with either siRNA against CRYAB or non-specific siRNA. Significant increase in caspase 3 activity was observed in hypoxic cardiomyocytes treated with siRNA against CRYAB. The siRNA treatment had no effect on caspase 3 activity during hypertrophy (**P<0.01)