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. 2013 Apr 18;4(4):e594. doi: 10.1038/cddis.2013.115

Figure 4.

Figure 4

FasL induced ERK1/2 phosphorylation and survivin upregulation in BM–MSCs. (a) BM–MSCs (batch no. 1) were treated with 0.5 ng/ml FasL for 5, 15, 30, or 60 min and cell lysates immunoblotted with anti-phospho-ERK1/2 (p-ERK1/2) and anti-total ERK (tot-Erk; upper panel). Protein levels were evaluated by densitometric analysis and the amount of p-ERK was expressed as a percentage of tot-ERK (lower panel). N=3. (b) Survivin expression in BM–MSCs, untreated (white bars) or treated with 0.5 ng/ml FasL (black bars), was analyzed on day 2, 4, and 6 of culture by western blotting (upper panel), quantified by densitometric analysis, and expressed as percentage of actin level (lower panel) N=3. Data are expressed as mean±S.D.