Figure 2.

p75^NTR and TrkB protein levels are also modified in the striatum of full-length Hdh^Q111/111 mutant mice. (a) Representative immunoblots showing the levels of p75^NTR, TrkB and β-actin as a loading control in striatal extracts obtained from wild-type Hdh^Q7/7 and mutant Hdh^Q111/111 mice at 1, 2 and 12 months of age. The histograms represent the relative levels of p75^NTR and TrkB expressed as percentage of wild-type values. Values are given as mean±S.E.M. of 5–6 independent samples. Data was analyzed by two-way ANOVA followed by Student's t-test. *P<0.05; **P<0.01; ***P<0.001 respect to wild-type mice. (b) (Upper panel) double immunostaining showing p75^NTR and DARPP32 in the striatum of wild-type (Hdh^Q7/7) and mutant (Hdh^Q111/Q111) mice at 12 months of age. Some cells are single-labeled for p75^NTR (arrows), whereas a high proportion of cells are double-labeled for p75^NTR and DARPP32 in both wild-type and HD mutant mice (arrowheads). (Middle panel) double immunostaining showing p75^NTR and parvalbumine staining in the striatum of mutant Hdh^Q111/111 mice at 12 months of age. Most of the parvalbumine-positive neurons are also positive for p75^NTR. (Bottom panel) Double immunostaining showing p75^NTR and GFAP in the striatum of mutant (Hdh^Q111/111) mice at 12 months of age. p75^NTR and GFAP immunoreactivity demonstrates lack of colocalization. Scale Bar, 25 μm