Figure 3.

Modestly reduced proliferation in CREB−ve intestinal crypts despite uniform epithelial KO. (a and b) PCNA IHC was performed and determined at each crypt position from the base, showing a modest reduction in proliferation in the transit-amplifying region of the colonic crypt (d and e) and throughout the SI crypt. (e) Overall, the total PCNA staining in the colon was not significantly different (f), whereas a statistically reduced number of PCNA+ve nuclei were found in the SI. (g) Relative levels of mRNA for CREB target gene pcna was found to be statistically reduced following KO. Means±S.E.M., two-tailed t-test. *P=0.01; ***P=0.001