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E6AP inhibition leads to granulocytic differentiation in 32Dcl3 cells. 32Dcl3 cells were transfected with siE6AP and E6AP-C843A and were induced with G-CSF for the indicated time points. After (a) 3 days, (b) 9 days and (c) 15 days of induction cells were washed, cytospun and stained with May-Grünwald and Giemsa staining (bold arrows=granulocytes/neutrophils; simple arrows=apoptotic cells)
