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. 2013 Mar 25;2(3):e39. doi: 10.1038/oncsis.2013.4

Figure 4.

Figure 4

Induction of EMT in EGFR TKI-resistant NSCLC cell lines is associated with induction of FGFR1. (a) Cell lysates from the indicated NSCLC cell lines resistant to 3 μℳ gefitinib or 2 μℳ BIBW2292 (R) or DMSO-treated passage control (d) were immunoblotted for E-cadherin, vimentin and the α subunit of the NaK-ATPase as a loading control. (b) HCC4006 DMS0 and gefitinib-resistant cells were plated in media containing 2% Matrigel and allowed to form colonies over 7 days. Colonies were imaged at × 100 magnification using a Nikon Eclipse TS100 camera. (c) HCC4006 control and gefitinib-resistant cells were treated 1, 3 or 6 days with DMSO or 0.3 μℳ AZ8010. Cell lysates were immunoblotted for E-cadherin and the α-subunit of NaK-ATPase as a loading control.