Figure 1.

Expression of human IF1 in normal and tumor tissues. Western blots reveal the expression of IF1 (h-IF1) in SDS–polyacrylamide gel electrophoresis fractionated proteins from normal (N) and tumor (T) biopsies of different human tissues. The antibody recognizes the recombinant protein, as well as the two major 12- and 8-kDa protein isoforms of IF1. β-actin expression is shown as loading control. For comparison purposes, the expression level of IF1 in human tissues (histograms) is normalized relative to its expression in HeLa cells (a.u., arbitrary unit) assayed in the same blot. The number of paired normal and tumor biopsies analyzed is indicated in parenthesis.