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. 2013 Apr 22;2(4):e46. doi: 10.1038/oncsis.2013.9

Figure 4.

Figure 4

IF1 regulates the activity of aerobic glycolysis in the lung (HOP62, A549), breast (BT549, HS578T) and ovarian (OVCAR8, OVCAR3) cancer cells. (a) Cells transfected with CDL-GFP-β-3′UTR were co-transfected with control (CRL and siCRL, open bars), IF1 plasmid (IF1+, dark gray bars) or siIF1 small interfering RNA (siIF1, light gray bars) to regulate the expression of IF1 for the determination of the rates of aerobic glycolysis. The effect of 6 μℳ oligomycin (OL, closed bars) is shown. Representative blots of IF1 and β-F1-ATPase (β-F1) expression. Bars are the mean±s.e.m. of six different samples. *P<0.05 when compared with CRL by Student's t-test. (b) IF1 (h-IF1) expression in 20 μg of protein from different human cell lines. Two different exposures of the IF1 film are presented. β-actin expression is shown as loading control.