Figure 5.

IF1 regulates mitochondrial ROS production in the lung (HOP62, A549), breast (BT549, HS578T) and ovarian (OVCAR8, OVCAR3) cancer cells. Cells transfected with CDL-GFP-β-3′UTR were co-transfected with control (CRL, open and light gray bars) or IF1 plasmid (IF1, dark gray and closed bars) in the absence or presence (+MQ) of 5 nℳ of the mitochondrial ROS scavenger MitoQ (MQ). The superoxide radical was determined by fluorescence-activated cell sorting analysis using MitoSox. The data shown are mean±s.e.m. of 12 different samples. *P<0.05 and ^#P<0.05 when compared with CRL or IF1 by Student's t-test, respectively.