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. 2013 Apr 22;2(4):e46. doi: 10.1038/oncsis.2013.9

Figure 7.

Figure 7

IF1 triggers transcriptional activation of the NFκB promoter in the lung (HOP62), breast (BT549) and ovarian (OVCAR8) cancer cells. Cells transfected with CDL-GFP-β-3′UTR were co-transfected with control (CRL, open and light gray bars) or IF1 (IF1, dark gray or closed bars) plasmid in the absence or presence (+MQ) of 5 nℳ of the mitochondrial ROS scavenger MitoQ (MQ). (a) A luciferase reporter plasmid of the NKκB promoter was co-transfected and the luciferase activity was determined in cellular extracts after 24-h transfection. The data shown are mean±s.e.m. of 10 samples. *P<0.05 and #P<0.05 when compared with CRL or IF1 by Student's t-test, respectively. (b) Cellular proteins of HOP62 were fractionated on SDS–polyacrylamide gel electrophoresis and processed for western blotting with the indicated primary antibodies. Representative blots are shown. The results are mean±s.e.m. of three experiments. *P<0.05 and #P<0.05 when compared with CRL or IF1 by Student's t-test, respectively.