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. 2012 Aug 29;21(5):517–521. doi: 10.1038/ejhg.2012.197

Figure 4.

Figure 4

Gene and protein expression levels 48 h after knockdown of YY1 using siRNA in MG63 cells. Left, YY1 gene expression when treated with YY1 siRNA compared with that of a non-targeting (NT) siRNA. Middle, GDF5 gene expression when treated with YY1 siRNA compared with that of a NT siRNA. Data shown are gene expression relative to that of the GAPDH housekeeper plus the SE of the mean. The YY1 expression data are presented as 2−(YY1-GAPDH) while the GDF5 expression data are presented as 2−(GDF5-GAPDH), with statistical analysis performed using a Student's t-test (***P<0.001, **P=0.0097). Right, western blot of YY1 protein from cells treated with either NT siRNA or a YY1 siRNA. β actin antibody was used as a protein housekeeper to confirm equal loading.