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. 2013 Mar 29;45(3):e16. doi: 10.1038/emm.2013.18

Figure 1.

Figure 1

Stable PIP5Kα KD in PC12 cells. (a) Stable PC12 cells expressing control miR or PIP5Kα miR were generated using a vector-based miR expression system harboring an EmGFP reporter. Stable cells and EmGFP were visualized in the bright field and fluorescein isothiocyanate channels, respectively, using fluorescence microscopy. Scale bar, 20 μm. (b) mRNA expression levels of PIP5Ks in control and PIP5Kα KD cells were examined by reverse transcription–PCR with their specific PCR primers. (c) PIP5Kα protein expression in control and PIP5Kα KD cells was analyzed by western blotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (b) or β-actin (c) was included as a loading control. (d) Changes in mRNA and protein expression levels of PIP5Kα in PIP5Kα KD cells were normalized to those in control cells. Values are presented as mean±s.e.m. *P<0.01.