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Effect of PIP5Kα KD on nerve growth factor (NGF)-induced neurite outgrowth. (a) Control and PIP5Kα KD cells were treated with or without NGF (100 ng ml−1) for 24 or 48 h. Bright field images of neurite outgrowth were obtained using an inverted microscope. Scale bar, 20 μm. (b) Lengths of the longest neurites were measured from more than 40 different cells in each group. Values are mean±s.e.m. *P<0.01. miRNA, microRNA.
