Figure 3.
Changes in nerve growth factor (NGF)-induced phosphatidylinositol (PI)3K/Akt activation by PIP5Kα KD. Control (a) and PIP5Kα KD (a, c) cells were treated with or without NGF (100 ng ml−1) for 15 min. (c) LY294002 (20 μℳ) was added for 1 h before NGF treatment as indicated. (a, c) Phosphorylated and total levels of Akt were measured by western blotting. (b) The phosphorylation levels of Akt by NGF in (a) were quantified as fold-change over those in NGF-stimulated control cells. Values are mean±s.e.m. *P<0.01. miRNA, microRNA.