Figure 1.
Turbidity-assessed time course of in vitro microtubule assembly. (a) Chemical structures of colchicine and CT20126. (b) Taxol, colchicine or CT20126 (5 nℳ each) were added to tubulin polymerizing buffer containing 0.1 μg μl−1 tubulin and 1 mℳ guanosine-5'-triphosphate (GTP; arrow ‘a'). After the samples were cooled on an ice bath for depolymerization, repolymerization was initiated by increasing the temperature to 37 °C (arrow ‘b'). DMSO, dimethyl sulfoxide. (c) Dose-response effects of CT20126 on microtubule polymerization. Arrows ‘a' and ‘b' indicate the drug treatment and rewarming times, respectively.