Figure 6.

Effects of ZD1839 on dopaminergic markers in the globus pallidus of epidermal growth factor (EGF) rats. (a) A protein extract was prepared from the globus pallidus of EGF rats receiving ZD1839 or vehicle (8-week old, n=6 rats each) and subjected to SDS-polyacrylamide gel electrophoresis. Immunoblots were probed with the antibodies directed against tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), dopamine transporter (DAT), DARPP32 and catechol-O-methyltransferase (COMT) as well as with those against neuron-specific enolase (NSE) and eukaryotic elongation factor 2 (eEF2; internal controls). Immunoreactivity on a blot was captured by a cooled CCD camera and measured by imaging software. (b) The percentage ratio normalized to control rats was calculated and plotted (mean±s.e.m.). *P<0.05, ***P<0.001 by two-tailed t-test.