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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Nature. 2011 Apr 3;472(7343):351–355. doi: 10.1038/nature09865

Figure 2. Neuronal activity is essential for the proper laminar migration of selective interneuron subtypes.

Figure 2

a. Laminar positioning of electroporated interneurons in wild type mice (control) and tetO-Kir2.1.ires.LacZ littermates both co-electroporated with Dlx5/6-Tta and Dlx5/6-eGFP plasmids at e15.5. Tbr1 expression delineates layers II/III and V at P5-P8. Representative examples taken from the analysis of 4 control and 6 tetO-Kir2.1.ires.LacZ electroporated mice for each developmental stage. b. Quantification of the distribution of VIP+, Cr+ and Re+ interneuron subtypes across cortical layers at P8. Due to the lack of selective molecular markers to distinguish between cortical layer II and III at P8-P9, we divided these layers collectively into II/IIItop (II/IIIt) and II/IIIbottom (II/IIIb). Mean percentage values (±SEM) were obtained from 4 wild type and 6 tetO-Kir2.1.ires.LacZ electroporated mice. Paired t-test: *, P<0.05; **, P<0.01; ***, P<0.001.