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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Nature. 2011 Apr 3;472(7343):351–355. doi: 10.1038/nature09865

Figure 3. Specific interneuron subtypes require activity for migration and morphological maturation at two distinct stages of development.

Figure 3

a. Laminar positioning of P8 electroporated interneurons in wild type mice (control) and tetO-Kir2.1.ires.LacZ mice both co-electroporated with Dlx5/6-Tta and Dlx5/6-eGFP plasmids at e15.5. Mice received either no treatment (Kir2.1on); or were treated with Dox at e16.5 (Kir2.1off @ P0 onwards); or with Dox at P0 (Kir2.1off @ P3 onwards). b. β-galactosidase activity in P8 tetO-Kir2.1.ires.LacZ mice co-electroporated with Dlx5/6-Tta and Dlx5/6-eGFP plasmids either untreated or treated with Dox at e16.5 (Kir2.1off @ P0 onwards). c. Neurolucida reconstructions of Cr+ and Re+ interneurons in wild type (control) and tetO-Kir2.1.ires.LacZ mice both co-electroporated with Dlx5/6-Tta and Dlx5/6-eGFP plasmids. Mice received either no Dox treatment (Kir2.1on) or Dox at P0 (Kir2.1off @ P3 onwards). Axons are shown in red, dendrites in blue and somata in black. Scale bar: 50 μm d. Quantification of dendritic and axonal morphology in control and experimental Cr+ and Re+ interneurons in tetO-Kir2.1.ires.LacZ mice after Dox administration at P0. Mean percentage values (±SEM) were obtained from >3 reconstructed interneurons each in Dox-treated wild type and tetO-Kir2.1.ires.LacZ mice for each subtype analyzed at P8.