Figure 5. POMGnT1 knockout neural sphere exhibited a reduced rate of accumulation of BM components.
Neural spheres were incubated with Matrigel for variable times and bound laminin-111 and collagen IV were visualized by immunofluorescence staining. (A–F) Wildtype. (A′-F′) POMGnT1 knockout. (G) Mean laminin-111 fluorescent intensity per μm2. (H) Mean collagen IV fluorescence intensity per μm2. (I) Average size of aggregates. (J) Average fluorescence intensity of aggregates. Scale bar in F′: 10 μm.