Table I.
Initial assembly rate (Vinitial) of ECM molecules on POMGnT1 KO neural stem cell cultures is reduced.
| Cell lines | Bmax | T1/2 | Vinitial (Mean ± SEM) | p value |
|---|---|---|---|---|
| laminin-111 overlay on monolayer cultures (Figure 2I) | ||||
| Wildtype + Ad-GFP | 4.853 | 0.545 | 5.605 ± 0.896 | |
| POMGnT1 KO+Ad-GFP | 2.073 | 5.046 | 0.859 ± 0.214 | <0.001 |
| Wildtype + Ad-POMGnT1 | 4.496 | 0.619 | 5.631 ± 0.672 | |
| POMGnT1 KO +Ad-POMGnT1 | 3.758 | 0.552 | 6.291 ± 0.428 | 0.442 |
| Laminin-111 overlay on neural sphere cultures (Figure 3G) | ||||
| Wildtype | 6.606 | 0.532 | 14.76 ± 1.772 | |
| POMGnT1 KO | 2.552 | 0.367 | 5.124 ± 0.926 | 0.002 |
| Matrigel overlay (laminin-111 and collagen IV double staining) (Figure 5G and H) | ||||
| Wildtype (laminin-111) | 11.47 | 0.895 | 15.53 ± 1.982 | |
| POMGnT1 KO (laminin-111) | 6.219 | 1.098 | 5.832 ± 1.925 | 0.024 |
| Wildtype (collagen IV) | 9.156 | 0.830 | 13.19 ± 2.223 | |
| POMGnT1 KO (collagen IV) | 4.840 | 1.015 | 5.646 ± 1.951 | 0.058 |
| Matrigel overlay (laminin-111 and perlecan double staining) (Supplementary Figure 4F and G) | ||||
| Wildtype (laminin-111) | 9.050 | 0.690 | 12.28 ± 0.990 | |
| POMGnT1 KO (laminin-111) | 5.385 | 0.548 | 8.900 ± 0.976 | 0.049 |
| Wildtype (perlecan) | 5.716 | 0.514 | 7.435 ± 1.045 | |
| POMGnT1 KO (perlecan) | 3.732 | 0.786 | 3.783 ± 0.886 | 0.047 |
Abbreviations: Bmax, maximal immunofluorescence intensity; KO, knockout; SEM, standard error of the mean; T1/2, time to reach 50% maximal immunofluorescence intensity; Vinitial, initial rate of fluorescence increase. All p values are comparisons of knockout samples with corresponding wildtype controls.