Table 1.
The site-specific PCR amplification protocols.
| Genes | Primers for PCR | PCR conditions | The length of amplified fragments (bp) | Restriction enzyme | Restricted products length and corresponding genotype | Reference |
|---|---|---|---|---|---|---|
| GSTM1 | s 5′-GAACTCCCTGAA AAGCTAAAG C-3′, as 5′-GTTGGGCTCAAATATACGGTGG-3′ | Initiation denaturation step at 94°C for 5 min, followed by 35 cycles of 94°C for 2 min, 59°C for 1 min, 72°C for 1 min and final elongation step at 72°C for 10 min. | 215 | Not used | – | Abbas et al. (2004) |
| GSTT1 | s 5′-TTCCTTACTGGTCC TCACATCTC-3′, as 5′-TCACCGGATCATGGCCAGCA-3′ | 480 | Not used | – | ||
 (as a internal control) |
s 5′-CCACTTCATCCACGTTCACC-3′, as 5′-GAAGAGCCTAGGACAGGTAC-3′ | 268 | Not used | – | ||
| XRCC1 Arg194Trp | s 5′-GCCCCGTCCCAGGTA-3′, as 5′-AGCCCCAAGACCCTTT-3′ | Initiation denaturation step at 95°C for 2 min, followed by 40 cycles of 94°C for 15 s, 57°C for 45 s, 72°C for 45 s and final elongation step at 72°C for 5 min | 490 | PvuII, 10 × Tango buffer | Arg/Arg – 490 bp; Arg/Trp – 490, 294, and 196 bp; Trp/Trp – 294 and 196 bp | Au et al. (2003) |
| XRCC1 Arg399Gln | s 5′-CAAGTACAGCCAGGTCCTAG-3′, as 5′-CCTTCCCTCATCTGGAGTAC-3′ | Initiation denaturation step at 95°C for 2 min, followed by 40 cycles of 94°C for 15 s, 55°C for 30 s, 72°C for 45 s and final elongation step at 72°C for 5 min | 248 | Bcn1, 10 × Tango buffer | Arg/Arg – 159 and 89 bp; Arg/Gln – 248, 159, and 89 bp; Gln/Gln – 248 bp | Au et al. (2003) |
| XRCC3 Trp241Met | s 5′-GCCTGGTGGTCATCGACTC-3′, as 5′-ACAGGGCTCTGGAAGGCACTGCTCAGC TCACGCACC-3′ | Initiation denaturation step at 94°C for 3 min, followed by 35 cycles of 95°C for 1 min, 60°C for 1 min, 72°Cfor 1 min and final elongation step at 72°C for 5 min | 136 | Nco1, 10 × Tango buffer | Trp/Trp – 136 bp, Trp/Met – 136, 97, and 39 bp; Met/Met – 97 and 39 bp | Au et al. (2003) |
| TP53 Arg72Pro | s 5′-TGAGGACCTGGTCCTCTGAC-3′, as 5′-AGAGGAATCCCAAAGTTCCA-3′ | Initiation denaturation step at 94°C for 2 min, followed by 35 cycles of 94°C for 30 s, 54°C for 30 s, 72°C for 30 s and final elongation step at 72°C for 5 min | 412 | Bsh1236I (Acc II), 10 × r buffer | Arg/Arg – 252 and 160 bp; Arg/Pro – 412, 252 and 160 bp; Pro/Pro – 412 bp | Lu et al. (2004) |