Remodeling/opening of 30-nm chromatin fiber in vitro: requirements for SWI/SNF and p300.
(A) Reaction scheme for analyzing NF1 binding to compacted chromatin template p(PEPCK-500)G. (B, C) DNase I footprinting analysis for NF1 binding using chromatinized template assembled with hypoacetylated histones and histone H1, as a function of the presence of the factors indicated at the top and also shown in the reaction scheme in panel (A). (B) The binding site of NF1 is indicated by a bond bar, and hypersensitive sites are indicated by red stars. Naked DNA is shown as control. (C) ATP and Ac-CoA are required for the remodeling/opening of compacted chromatin mediated by SWI/SNF and p300 to facilitate NF1 binding. See also Figure S3.