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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Mol Cell. 2010 Apr 9;38(1):41–53. doi: 10.1016/j.molcel.2010.01.042

Figure 3.

Figure 3

Remodeling/opening of 30-nm chromatin fiber in vitro: requirements for SWI/SNF and p300.

(A) Reaction scheme for analyzing NF1 binding to compacted chromatin template p(PEPCK-500)G. (B, C) DNase I footprinting analysis for NF1 binding using chromatinized template assembled with hypoacetylated histones and histone H1, as a function of the presence of the factors indicated at the top and also shown in the reaction scheme in panel (A). (B) The binding site of NF1 is indicated by a bond bar, and hypersensitive sites are indicated by red stars. Naked DNA is shown as control. (C) ATP and Ac-CoA are required for the remodeling/opening of compacted chromatin mediated by SWI/SNF and p300 to facilitate NF1 binding. See also Figure S3.