Figure 5.

mSSB1 and mSSB2 protect both leading and lagging G-overhangs. (A) Examples and schematics illustrating the various chromosome and chromatid fusions observed in the absence of mSSB1 or both mSSB1 and mSSB2. (B, C) Quantification of chromatid fusions (treated with TPP1^ΔRD) in WT or mSSB1^Δ/Δ MEF (B) or mSSB1^Δ/Δ MEFs reconstituted with WT or mSSB1 mutants (C). Mean values were derived from three independent experiments. P values were calculated by one-tailed t-test. Error bars: s.e.m. (D) Detection of G-overhang (native) and total telomere length (denature) in reconstituted mSSB1^Δ/Δ MEFs by TRF Southern blot. Numbers under the native gel indicate the relative ratios of G-overhang to total telomere signal. γ-³²P- (CCCTAA)₄ telomere probe was used for both native and denature gel hybridizations. Molecular weight markers are indicated.