Figure 6.

DUOX2 regulates secreted levels of type I/III IFNs at the late stages of viral infection. (A) A549 cells were transfected with siCTRL, siDUOX2(1) or siDUOX2(2), and 48 h post-transfection, cells were infected with SeV (40 HAU/10⁶ cells) for the indicated times. DUOX2 expression was analyzed by immunoblot analyses using DUOX1/2 specific antibodies. Release of IFNβ, IFNλ and TNFα was measured by multiplex ELISA. (B) A549 cells were transfected with siCTRL or siDUOX2(2) for 48 h and infected as in A for the indicated times. IFNβ, IFNλ (IL-28A and IL-29) and TNFα mRNA absolute copy numbers were analyzed by qRT-PCR. Data were analyzed by two-way ANOVA with Bonferroni post-test. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. The dotted line in qRT-PCR graphs represents the threshold of detection.