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TM regions evolve more slowly than EM regions. When residues from membrane proteins are binned broadly according to the type of region in which they lie (EM vs. TM), we observe that residues from TM regions (red) tend to evolve much more slowly than their counterparts in EM regions (blue), as measured by dN/dS. Error bars reflect estimates of the standard error from 100 rounds of bootstrap resampling.
