Figure 6. Antagonism of miR-133 during Muscle Regeneration Reduced Adiposity, Increased Total Energy Expenditure, and Improved Glucose Tolerance.

(A) Representative images of direct comparisons of C57BL/6 mice fed the RD or HFD and received control or miR-133 ASO treatment during TA muscle regeneration depict that the miR-133 ASO-treated mice displayed leaner phenotype.

(B) Retarded body weight increase over a 16 week body weight monitoring time course in mice that received miR-133 ASO treatment during TA muscle regeneration (n = 6 per treatment group per diet group).

(C) miR-133 ASO treatment increased total energy expenditure. Indirect calorimetry reveals that mice receiving miR-133 ASO treatment during TA muscle regeneration had a recorded higher energy expenditure than the control mice (n = 5 per group), measured at 22°C during light and dark cycles (fed the RD). Values of total energy expenditure were plotted without normalization to lean body mass.

(D) Physical activities measured within light and dark cycles during indirect calorimetry (C) reveal increased physical activities within the dark cycle for miR-133 ASO-treated mice (fed the RD). Physical activities are presented as arithmetic means of beam-breaking events at X, Y, and Z dimensions.

(E) IPGTT tests for C57BL/6 mice received either control or miR-133 ASO treatment (fed the RD; n = 6 per group).

Error bars, SEM. See also Figure S6.