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. Author manuscript; available in PMC: 2014 Feb 5.
Published in final edited form as: Cell Metab. 2013 Feb 5;17(2):210–224. doi: 10.1016/j.cmet.2013.01.004

Figure 7. Cold Exposure Downregulates miR-133 Expression in Skeletal Muscles.

Figure 7

(A) Cold exposure induced downregulation of miR-133 (miR-133a and miR-133b) in multiple adipose and muscle tissues and increased expression of brown adipocyte markers, Prdm16 and Ucp1, in subcutaneous WAT and trunk muscles. C57BL/6 mice were raised under ambient temperature (23°C) or exposed to cold (4°C) for 1 week. Multiple tissues were dissected and investigated by RT-qPCR for the expressions of miR-133, Prdm16, and Ucp1.

(B) Cold exposure induced satellite cells to differentiate into brown adipocytes located within the muscle interstitium in paraspinal muscles. Pax7-CreER;R26R-tdTomato mice were raised under ambient temperature (23°C) or exposed to cold (4°C) for 1 week. Representative images of paraspinal muscle cross-sections stained with DAPI, Ucp1, and Laminin together with tdTomato native fluorescence revealed SC_BAs within the muscle interstitium.

Error bars, SEM.