|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| Milli-Q water | 44 | |
| NEbuffer 2 (10×) | 10 | 1× |
| S-adenosylmethionine (32 mM) | 2 | 0.64 mM |
| Unmethylated λ-DNA (450 μg ml−1) | 40 | 180 ng μl−1 |
| SssI methylase (20 U μl−1) | 4 | 0.8 U μl−1 |
| Final volume | 100 | |
|
| ||
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