|
| ||
| Component | Amount | Final concentration |
|
| ||
| Genomic DNA of interest | 500 ng | 10 ng μl−1 |
| Methylated λ-DNA (from Step 3 of the main PROCEDURE) | 2.5 ng | |
| Tet oxidation reagent 1 | 3.5 μl | |
| Tet oxidation reagent 2 | 15 μl | |
| mTet1 protein (3 mg ml−1) | 5 μl | 0.3 μg μl−1 |
| Milli-Q water | to 50 μl | |
| Final volume | 50 μl | |
|
| ||
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