|
| ||
| Component | Amount (μl) | Final concentration |
|
| ||
| PfuTurbo Cx reaction buffer (10×) | 5 | 1× |
| dNTP mix (25 mM each) | 0.4 | 200 μM each |
| 5-mC_test_F (10 μM) | 1 | 0.2 μM |
| 5-mC_test_R (10 μM) | 1 | 0.2 μM |
| Bisulfite-treated DNA (from step 5) | 2 | |
| PfuTurbo Cx DNA polymerase (2.5 U μl−1) | 1 | 2.5 U per 50 μl PCR |
| Milli-Q water | to 50 | |
| Final volume | 50 | |
|
| ||
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