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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Nat Protoc. 2012 Nov 29;7(12):2159–2170. doi: 10.1038/nprot.2012.137
Cycle number Denature Anneal Extend
1 95 °C, 10 min
2–41 95 °C, 30 s 57 °C, 30 s 72 °C, 1 min for tube 2;
72 °C, 1.5 min for tube1
42 72 °C, 7 min