|
| ||
| Component | Amount | Final concentration |
|
| ||
| Sheared DNA from Step 11 | 1 or 3 μg | 50 or 150 ng μl−1 |
| UDP-glucose (10 mM) | 1μl | 200 μM |
| β-GT protection buffer (10×) | 2μl | 1× |
| T4-βGT (40 μM) | 0.5 μl | 1 μM |
| Milli-Q water | to 20 μl | |
| Final volume | 20 μl | |
|
| ||
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