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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Dev Biol. 2013 Jan 25;377(1):275–283. doi: 10.1016/j.ydbio.2013.01.019

Figure 6.

Figure 6

Deletion of the GNARLE increases protein synthesis. Synthetic mRNAs, containing Renilla luciferase (Rluc) ORF preceded by (A) Xenopus β-globin 5’UTR or (B,C,D) Sp nanos2 5’UTR and followed by (A) Xenopus β-globin 3’UTR, (B) Sp nanos2 full length 3’UTR, (C) Sp nanos2 GNARLE 3’UTR, or (D) Sp nanos2 ΔGNARLE 3’UTR, were injected in Sp fertilized eggs. An mRNA containing the Firefly luciferase (Fluc) ORF flanked by Xenopus β-globin 5’ and 3’UTRs was co-injected. Luminescence was measured at mesenchyme blastula. The ratio Rluc/Fluc was determined, and the results are shown in percentages considering the ratio obtained for Sp ΔGNARLE (D) as 100%. Error bars indicate the standard deviation from three technical replicates.