(A) Representative force tracings of perfused (solid line) and ischemic (grey line) mesenteric microvessels during 80mM KCl depolarization. Following KCl depolarization, force rapidly rises to a peak in both perfused and ischemic preparations. However, force maintenance was reduced in the ischemic mesenteric vessels, compared to that in perfused vessels (P<0.05). (B) Representative force tracings of perfused (solid line) and ischemic (grey line) mesenteric microvessels during PE activation. Force rapidly rises to a peak in both perfused and ischemic preparations. However, force maintenance was reduced in the ischemic mesenteric vessels, as compared to that in perfused vessel (P<0.05).