Table 1.

PCR primers used to synthesize the porcine CD3 molecules.

For porcine CD3ε ectodomain

pEP1

5′G GAA TTC CAT ATG GAG GAC ATT GAG AGA CCA GAC GAG GAC ACT CAA AAG ACT TTC AAG 3′

pEP2

5′ACA AGT CAA CTC AAC CTT GTC ACC AGA AAT AGA AAC CTT GAA AGT CTT TTG AGT GTC 3′

pEP3

5′GAC AAG GTT GAG TTG ACT TGT CCA GAG GAC CCA GAG TCT GAG AAG ATG ACT TGG AAG 3′

pEP4

5′GTT GTC GTA AGA CTC GTA AAT TTG CAT GTC GTT TCT CTT CCA AGT CAT CTT CTC AGA 3′

pEP5

5′ATT TAC GAG TCT TAC GAC AAC TAC ATG TTG TTG GAG AGA TTC TCT GAG GTT GAG AAC 3′

pEP6

5′GTG CTT CTC ACC AAC AGT ACA AGT GTA GTA ACC AGA GTT CTC AAC CTC AGA GAA TCT 3′

pEP7

5′TGT ACT GTT GGT GAG AAG CAC ACT CAC AGA TTG TAC TTG AAG GCT AGA GTT TGT GAG 3′

pEP8

5′CCG GAA TTC TTA GTC AAC CTC AAC ACA GTT CTC ACA AAC TCT AGC CTT CAA 3′

For porcine CD3γ ectodomain

pGA1

5′G GAA TTC CAT ATG CAA TTG AAG GAG GGT AAG CAC TCT GTT TTG TTG GAC GAC AAC 3′

pGA2

5′CAA ACC ACA AGT CAA CAA AAC AGA ACC GTC CTC TCT GTT GTC GTC CAA CAA AAC AGA 3′

pGA3

5′GTT TTG TTG ACT TGT GGT TTG CCA GAC CAA AAC ATT AGA TGG TTC AAG GAC GGT AAG 3′

pGA4

5′AGT AGA TCT AGA GTT GTT CAA AGA ACA AAT CTC CTT ACC GTC CTT GAA CCA TCT 3′

pGA5

5′TTG AAC AAC TCT AGA TCT ACT TGT AAC TTG GGT TCT TCT TCT AAG GAC CCA AGA 3′

pGA6

5′GTT CTC CTT AGA ACC CTC ACA CCA GTA AAT ACC TCT TGG GTC CTT AGA AGA AGA 3′

pGA7

5′TGT GAG GGT TCT AAG GAG AAC TCT AAG AGA TTG CAA GTT TAC TAC AGA ATG TGT 3′

pGA8

5′CCG GAA TTC TTA GTT CAA CTC AAT ACA GTT TTG ACA CAT TCT GTA GTA AAC TTG 3′

For porcine CD3δ ectodomain

pDE1

5′G GAA TTC CAT ATG GCT TTC TTG TCT AGA GTT TCT CCA TAC GAG GTT GAG ATG GAG 3′

pDE2

5′GTT ACA AGA AAC GAA AAC CTT GTC CTC CAA CTC CTC CAT CTC AAC CTC GTA TGG 3′

pDE3

5′AAG GTT TTC GTT TCT TGT AAC ACT TCT ATT ATT TGG TTG CAA GGT ACT GAG GGT 3′

pDE4

5′ACC CAA GTC AAT CTT CTT GTC AGA CAA CAA CTC ACC CTC AGT ACC TTG CAA CCA 3′

pDE5

5′GAC AAG AAG ATT GAC TTG GGT AAG AGA ATT TTG GAC CCA AGA GGT TTG TAC AAG 3′

pDE6

5′AGA GTT AGA GTC TTG CTC CTT TGG AGC GTT ACA CTT GTA CAA ACC TCT TGG GTC 3′

pDE7

5′AAG GAG CAA GAC TCT AAC TCT AAG ATT TTC TTG CAA GTT TAC TAC AGA ATG TGT 3′

pDE8

5′CCG GAA TTC TTA GTC CAA CTC AAC ACA GTT TTG ACA CAT TCT GTA GTA AAC TTG 3′

For porcine CD3εγ or εδ ectodomain single-chain fusion construct

Ep-NdeI

5′G GAA TTC CAT ATG GAG GAC ATT GAG AGA CCA GAC 3′

Epsilon R

5′CGC GGA TCC ACC ACC ACC AGA ACC ACC ACC ACC GTC AAC CTC AAC ACA GTT CTC 3′

Gamma F1

5′CGC GGA TCC GGT GGT GGT GGT TCT CAA TTG AAG GAG GGT AAG CAC 3′

Gamma Rhis

5′CCG GAA TTC TTA GTG GTG GTG GTG GTG GTG GTT CAA CTC AAT ACA GTT TTG 3′

Delta F

5′ GGA AGA TCT GGT GGT GGT GGT TCT GCT TTC TTG TCT AGA GTT TCT 3′

Delta Rhis

5′ CCG GAA TTC TTA GTG GTG GTG GTG GTG GTG GTC CAA CTC AAC ACA GTT TTG 3′