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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Eur J Immunol. 2012 Apr;42(4):870–879. doi: 10.1002/eji.201142054

Figure 3. M.tb burden in vaccinated CBA/J mice.

Figure 3

Mice were injected subcutaneously with PBS, adjuvant (Adj), Ag85 plus Adj, or M. bovis BCG. At least 10 weeks after the first injection, mice were challenged with 50–100 CFU of M.tb Erdman by aerosol, and euthanized 28 days later. Lungs (A) and mediastinal lymph nodes (B) were homogenized, diluted, and plated onto supplemented 7H11 agar plates. M.tb CFU were counted after 21 days at 37°C. Protection in the lungs (C) and mediastinal lymph nodes (D) was calculated by subtracting the Log10 CFU from vaccinated groups from the average Log10 CFU of the PBS group. Results are the average ± SEM of 2 independent experiments. Each experiment had 5 mice per group, total n = 10 mice per group. Data were analyzed by one-way ANOVA with Tukey’s posttest, *p<0.05, **p<0.01, ***p<0.001.