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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Eur J Immunol. 2012 Apr;42(4):870–879. doi: 10.1002/eji.201142054

Figure 4. Lung cells in vaccinated CBA/J mice.

Figure 4

Mice were injected subcutaneously with PBS, adjuvant (Adj), Ag85 plus Adj, or M. bovis BCG. At least 10 weeks after the first injection, mice were challenged with 50–100 CFU of M.tb, and euthanized 28 days later. Lung cells were fixed and labeled with anti-CD3, anti-CD4, anti-CD8, anti-CD44, anti-CD11c, and anti-MHCII. The total lung cells (A), absolute numbers of T cells (B), CD4 T cells (C), CD8 T cells (E), CD44hi (activated) T cells (D, F), CD11c+ cells (G) and mean fluorescent intensity of MHCII expression (H) were determined. Results are the average ± SEM of 2 independent experiments. Each experiment had 5 mice per group, total n = 10 mice per group. Data were analyzed by one-way ANOVA with Tukey’s posttest, *p<0.05, **p<0.01, ***p<0.001.