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(A) EIC of m/z 548.2 from the BMO pool showing two main isomers. (B) EIC of m/z 548.2 after digestion with α N-acetylgalactosaminidase. (C) EIC of m/z 548.2 after digestion with β N-acetylhexosaminidase. (D) Magnification on base line of m/z 548.2 from the BMO pool showing additional isomers. (E) Isomer-specific fragmentation of EIC m/z 548.2 at 12.5 min, confirming reducing end HexNAc.
