. Author manuscript; available in PMC: 2013 May 2.

Published in final edited form as: J Am Chem Soc. 2011 Apr 27;133(19):7361–7375. doi: 10.1021/ja108118v

Table 8.

Comparison of ITC-derived thermodynamic profiles of 3 and neomycin with d[5’- A₃G₅C₅T₃-3’]. Buffer conditions = 100 mM KCl, 10 mM SC, 0.5 mm EDTA, pH 6.8. T = 25 °C. [DNA] = 4 μM/duplex. [3] = 125 μM. [neomycin] = 250 μM.

Ligand:DNA	N₁	K₁ (M^-1)×10⁵	ΔH₁(Kcal/mol)	TΔS₁ (Kcal/mol.K)
3:D4	2.64±0.09	7.75±1.70	-3.19±0.01	1.43
Neomycin:D4	0.94±0.20	2.02±0.20	-11.88±0.32	-5.45
Neomycin:D1	1.50±0.04	3.80±0.18	-6.89±0.23	0.53