Skip to main content
. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Cancer Res. 2008 May 15;68(10):3697–3706. doi: 10.1158/0008-5472.CAN-07-6702

Figure 5.

Figure 5

Lowering SRC-3 levels in MMTV-Neu mice reduces epithelial cell proliferation and the expression of cyclins D1 and E. A and B, representative immunohistochemistries of (A) PCNA and (B) cyclin D1 expression in mammary gland tissue sections (6 mo of age) and representative mammary tumors from Neu/SRC-3wt and Neu/SRC-3+/− mice. Arrows, positive staining in mammary ductal epithelial cells (magnification, 40×). C, bar charts represent the quantification of PCNA and cyclin D1 immunohistochemical staining; 100 cells were counted per field, and 10 fields were counted per mouse (magnification, 40×). Columns, mean of three independent experiments; bars, SD (n = 3 mice from each indicated genotype). **, P < 0.001, one-way ANOVA. D, representative Western blot analysis from whole cell extracts prepared from cultured nontumorigenic mammary epithelial cells (3 mo of age) or mammary tumor cells, for cyclin D1 and cyclin E expression. Densitometry values represent cyclin D1 and cyclin E levels normalized to total actin. Western blot results represent data from at least two mice from each genotype.