CD scans for the poly(dA)•poly(dT) duplex and poly(dA)•2poly(dT) triplex in the absence and presence of 2 (BQQ-neomycin). [a]= poly(dA)•2poly(dT) triplex (no ligand); [b]= poly(dA)•2poly(dT) triplex (0.13 equivalent 2); [c]= poly(dA)•poly(dT) duplex (no ligand); [d]= poly(dA)•poly(dT) duplex (0.13 equivalent 2). Experimental conditions: sodium cacodylate (10 mM, pH 5.5), EDTA (0.5 mM), KCl (150 mM), Temperature = 10°C.
b. Continuous variation scans (left) and A260 plot (right) of dA30 (1 µM/base) and dT30 (1µM/base) in the absence of drug (top) and presence of napthalenediimide-neomycin 3 (rbd =7) (bottom). Experimental conditions: sodium cacodylate (10 mM, pH 5.5), EDTA (0.5 mM), KCl (150 mM), 15°C.