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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Biochemistry. 2010 Jul 6;49(26):5540–5552. doi: 10.1021/bi100071j

Table 3.

Thermodynamic profiles of intercalator-neomycin conjugates (1–4) and intercalator 7 with poly(dA)•2poly(dT) at pH 5.5. Experimental condition: sodium cacodylate (10 mM), EDTA (0.5 mM), KCl (150 mM).

Ligand [KCl]


mM		 ΔHwca

(kcal/

mol)		 Tm0a

(°C)		 Tma

(°C)		 nb ΔH(10°C)c


(kcal/mol)		 ΔH(15°C)c


(kcal/mol)		 ΔH(20°C)c


(kcal/mol)		 ΔCpd


(cal/mol.K)		 KT(20°C)e


(M−1)
Neomycin 140 1.70 31.4 34.4 6.8 −0.6±0.1
(12°C)		 - −1.31±0.02 −87±4 (2.4±0.1)×105
1 150 1.67 33.8 49.5 7 −1.5±0.1 - −1.64±0.02 −10±3 (1.9±0.1)×106
3 150 1.67 33.8 55.4 7.5 −2.4±0.1 −2.6±0.1 −2.8±0.1 −40±20 (5.5±0.3)×106
4 150 1.67 33.8 64.0 7.5 −5.4±0.2 −6.1±0.2 −6.4±0.1 −110±15 (3.7±0.1)×107
2 150 1.67 33.8 68.8 7.5 −7.5±0.4 −8.5±0.4 −9.7±0.2 −220±20 (2.7±0.3)×108
7 150 1.67 31.0 37.2 4 *−1.4±0.1 −0.38±0.07 −0.81±0.01 −131 (1.89)×105
*

ΔH was calculated at 23°C.