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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Biochemistry. 2010 Jul 6;49(26):5540–5552. doi: 10.1021/bi100071j

Table 4.

Thermodynamic profiles of intercalator-neomycin conjugates (1–4) with poly(dA)●2poly(dT) at pH 6.8. Experimental condition: sodium cacodylate (10 mM), EDTA (0.5 mM), KCl (150 mM).

Ligand [KCl]

mM		 ΔHwca

(kcal/mol)		 Tm0a

(°C)		 Tma

(°C)		 nb ΔH(10°C)c


(kcal/mol)		 ΔH(20°C)c


(kcal/mol)		 ΔH(5°C)c


(kcal/mol)		 ΔCpd


(cal/mol.K)		 KT(20°C)e


(M−1)
Neomycin 150 1.59 34.4 49.5 6.5 −6.4±±0.1 −7.5±0.2 −8.2±0.04 −116±4 -
1 150 1.59 34.4 58.0 7 −3.2±0.1 −3.7±0.1 - −45±1 -
3 150 1.59 34.4 65.0 7.5 −6.8±0.1 −7.8±0.1 −8.3±0.8 −104±10 -
4 150 1.59 34.4 68.0 7.5 −1±0.9±0.2 −12.7±0.2 −14.5±0.1 −231±6 -
2 150 1.59 34.4 85.0 7.5 −15.9±0.4 −19.3±0.4 −21.1±0.4 −346±13 -