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. Author manuscript; available in PMC: 2014 Apr 17.
Published in final edited form as: Cell Host Microbe. 2013 Apr 17;13(4):406–416. doi: 10.1016/j.chom.2013.03.003

Figure 3. A high-throughput screen identified 18 compounds that rescue LK, see also Table S2.

Figure 3

(A) Fluorescence micrographs of each well of a 384-well plate with small molecules screened for curing in this assay. Columns outlined in blue and red are gentamicin (positive) and DMSO (negative) controls, respectively. Circled in orange are three compounds that showed significant rescue, as demonstrated by the absence of stained worms. (B) A scatter plot shows the percent survival in each of the two replicates for the example plate. Circles colored red represent wells containing DMSO, circles shaded blue depict gentamicin wells, and test compounds are shown as orange-colored circles. Three compounds showing significant rescue are at the upper right. (C) A list of the compounds, and the classes to which they belong, is also shown. (D) Dose-dependence of rescue by chelating molecules was tested. Ciclopirox olamine (CPX), 1,10-phenanthroline, or 2,2′-bipyridine were added to LK assay in serial two-fold dilutions and death was measured after 48 hours of infection. Error bars represent the standard error of three biological replicates.