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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: J Biochem Pharmacol Res. 2013 Feb 26;1(1):30–42.

Fig. 3.

Fig. 3

Serum DLDH dehydrogenase activity measured spectrophotometrically. (A) Analysis of serum samples derived from three mammalian species: rat, human, and mouse. The final assay mixture (1 ml) contained 5 mg serum protein, 3 mM dihydrolipoamide, 3 mM NAD+, 1.0 mM EDTA, 6 mg BSA, and 100 mM potassium phosphate (pH 8.0). The reaction was initiated by the addition of dihydrolipoamide and an absorbance increase at 340 nm was followed for 5 min for each sample. Serum DLDH activity shows dependence on both substrate concentration (B) and serum protein amount (C). For each point in B, 50 μl of serum was used; while for each point in C, 2.5 mM dihydrolipoamide was used. For both B and C, rat serum was used and enzyme activity was measured by the INT/PMS/NAD method [44] as described in the text with recording of absorbance at 505 nm.