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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: J Biochem Pharmacol Res. 2013 Feb 26;1(1):30–42.

Fig. 6.

Fig. 6

(A) Partial purification of DLDH from rat liver mitochondria assessed by SDS-PAGE, and (B) inhibition of its dehydrogenase activity by peroxynitrite. Unlike serum DLDH that lost its dehydrogenase activity after procedures of further isolation and purification, mitochondrial DLDH still remained active after the same procedures of isolation and purification, and the activity could only be purposely attenuated by incubation with peroxynitrite (1 mM). GSH (10 mM) was added after peroxynitrite incubation but before enzyme assay. *p<0.05 indicates statistical significance compared with control (Welch’s t test).