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Sca1-positive cells are recruited to tumor spheroids and are not proliferating. (A) Schematic of experimental design. Flow cytometry shows that 69% of Sca-1-positive cells are recruited (eGFP+) while 31% are proliferative (eGFP−). (B) Day 21 tumor spheroids were pulse-labeled with BrdU and immunostained for BrdU incorporation and Sca-1. BrdU+ cells (green) were not Sca-1-positive (red).
