Table 1.
PCR-RFLP conditions for OPRM1 gene polymorphisms
| SNP | Primer | Base change | Annealing temperature | Restriction enzyme |
|---|---|---|---|---|
| rs1799971 |
5’GTCTCGGTGCTCCTGGCTACCTCGC3’(F) |
A/G |
65 |
BsiEI |
| |
5’TTCGGACCGCATGGGTCGGACCGGT3’(R) |
|
|
|
| rs2075572 |
5’TAAGTTAGCTCTGGTCAAGGCTAAGAAT3’(F) |
C/G |
55 |
HinfI |
| |
5’ATCATCAGTCCATAGCACACGGTAA3’(R) |
|
|
|
| rs648893 |
5’AACAGATTAGGTCATTCTCACTTTA3’(F) |
C/T |
50 |
Bse3DI |
| |
5’GCTTTAGCATAATAGTGCCAGTTCC3’(R) |
|
|
|
| rs671531 |
5’ATCTGGCTAAGGCATCATTTTCACC3’(F) |
A/G |
53 |
SsiI |
| 5’TTTCACATCCAAGTAACTACACAGG3’(R) |