Table 3.
Nodulation [nodule number (NN) and dry weight (NDW)], plant growth [shoot and root dry weight (SDW, RDW)], N concentration (NC) and total N accumulated in shoots (TNS) of soybean cultivar BRS 245 inoculated or not with Bradyrhizobium japonicum CPAC 15 + B. diazoefficiens CPAC 7 and supplemented or not with genistein (5 μM) or concentrated metabolites (CM) of B. diazoefficiens USDA 110 (BD1) or Rhizobium tropici CIAT 899 (RT1)
|
Treatment |
NN |
NDW |
SDW |
RDW |
NC |
TNS |
|---|---|---|---|---|---|---|
| (# pl–1) | (mg pl–1) | (g pl–1) | (g pl–1) | (mg N g–1) | (mg N pl–1) | |
| Non-inoculated control |
zero2 |
zero |
0.81 b |
0.51 b |
6.57 b |
5.19 b |
| Inoculated with Bradyrhizobium |
38.0 b |
162 a |
1.31 a |
0.77 a |
19.38 a |
25.5a |
| Inoculated + genistein |
45.2 a |
169 a |
1.29 a |
0.72 a |
21.28 a |
28.3 a |
| Inoculated + CM-BD1 (1 mL L–1)1 |
45.9 a |
180 a |
1.43 a |
0.84 a |
21.57 a |
29.3 a |
| Inoculated + CM-BD1 (0.1 mL L–1) |
42.5 a |
140 a |
1.22 b |
0.63 ab |
19.07 a |
23.7 a |
| Inoculated + CM-RT1 (1 mL L–1) |
33.2 b |
138 a |
1.23 b |
0.66 ab |
20.65 a |
25.3 a |
| Inoculated + CM-RT1 (0.1 mL L–1) | 32.5 b | 128 a | 1.14 a | 0.61 ab | 19.81 a | 22.9 a |
1 0.1 and 1.0 mL L–1 correspond to approximately 10–9 and 10–8 M of active compounds, respectively.
2 Data represent the means of six replicates and when followed by different letters within the same column are significantly different (p ≤ 0.05, Duncan’s test).
Experiment performed in Leonard jars, with sterile substrate and receiving N-free nutrient solution, under greenhouse conditions. Plants were harvested at 45 days after emergence.